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實驗介紹：

FISH探針是指能與特定核酸序列發生特異性互補雜交，雜交后又能被特殊方法檢測的已知并被標記的核苷酸序列。FISH探針通過標記上熒光染料標記，后續用于可視化目標DNA序列的位置和數量。在確定目的基因序列后，選擇與目的基因序列互補的序列片段作為探針序列，并根據實驗條件選擇合適的熒光標記物進行標記。

探針類型：

①短鏈/寡核酸探針（15-40bp）是利用熒光染料標記到單鏈DNA上，通常使用化學合成方法，這可以通過磷酸化修飾或末端標記的方法將熒光染料引入到DNA分子的5' 末端或內部；

②長鏈探針(150-400bp）是將DNA分子的一端進行熒光染料標記，形成互補鏈5' 端帶有熒光標記的DNA雙鏈，通常使用PCR的方法進行標記將熒光染料引入到DNA分子其中一條鏈的5' 端。

標記類型：5' 端FAM/CY3/CY5等熒光標記。

規格：30T/50T/100T

文章案例：

Article：Streamlined metal-based hydrogel facilitates stem cell differentiation, extracellular matrix homeostasis and cartilage repair in male rats.

Periodicals：Nature Communications

IF：15.700

Article：Engineered EVs from LncEEF1G - overexpressing MSCs promote fibrotic liver regeneration by upregulating HGF release from hepatic stellate cells.

Periodicals：Experimental & Molecular Medicine

IF：9.500

Article：circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways. 

Periodicals：Molecular therapy. Nucleic acids

IF：6.500

Article：AQP3 Influences Unexplained Recurrent Abortion by Regulating Trophoblast Cell Migration and Invasion via the METTL14/IGF2BP1/AQP3/PI3K/AKT Pathway. 

Periodicals：Journal of Cellular and Molecular Medicine

IF：4.200

Article：m6A-modified exosome-derived circHIF1α binding to KH domain of IGF2BP3 mediates DNA damage and arrests G1/S transition phase to resists bacterial infection in bacteremia.

Periodicals：Journal of Nanobiotechnology

IF：10.600

Article：TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway.

Periodicals：The Clinical Respiratory Journal

IF：29.500

Article：Up-regulation of lncRNA CASC9 promotes esophageal squamous cell carcinoma growth by negatively regulating PDCD4 expression through EZH2.

Periodicals：Molecular cancer

IF：27.700

Article：Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1.

Periodicals：Clinical and translational medicine

IF：7.900