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實(shí)驗(yàn)原理：

將生物素標(biāo)記的miRNA探針轉(zhuǎn)染到細(xì)胞中，使細(xì)胞裂解物中的生物素標(biāo)記miRNA-RNA復(fù)合物能夠被鏈霉親和素磁珠高效富集，從而與裂解液中其他成分分離；隨后，通過(guò)洗脫純化得到RNA并進(jìn)行高通量測(cè)序或qPCR檢測(cè)，驗(yàn)證miRNA的靶基因。此外，還可以通過(guò)洗脫純化獲得結(jié)合在RNA上的蛋白并進(jìn)行質(zhì)譜檢測(cè)或Western blot檢測(cè)。

 

產(chǎn)品優(yōu)勢(shì)：

1. 高品質(zhì)，穩(wěn)定性好。

2. 易用：操作簡(jiǎn)單，容易上手。

3. 精準(zhǔn)：特異性高，背景少。

技術(shù)流程：

結(jié)果實(shí)例：

詳見(jiàn)產(chǎn)品說(shuō)明書(shū)：Bes5108 miRNA pulldown-RNA

詳見(jiàn)產(chǎn)品說(shuō)明書(shū)：Bes5109 miRNA pulldown-Protein

文章案例

【2025年】

Article：miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage

Periodicals：iScience

IF：4.100

Article：LncRNA TMC3-AS1 silence alleviates lipopolysaccharide-induced acute kidney injury by suppressing Wnt5a-mediated autophagy and pyroptosis pathway. 

Periodicals：Molecular and Cellular Probes

IF：2.300

Article：MicroRNA-18b-5p Inhibits the Malignant Progression of Prostate Cancer Through Downregulating TRAF5.

Periodicals：International Journal of General Medicine

IF：2.100

【2024年】

Article： Targeting piRNA-137463 Inhibits Tumor Progression and Boosts Sensitivity to Immune Checkpoint Blockade via De Novo Cholesterol Biosynthesis in Lung Adenocarcinoma. 

Periodicals：Advanced science (Weinheim, Baden-Wurttemburg, Germany)

IF：14.100

Article：A tumor suppressor protein encoded by circKEAP1 inhibits osteosarcoma cell stemness and metastasis by promoting vimentin proteasome degradation and activating anti-tumor immunity.

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：CircZCCHC2 decreases pirarubicin sensitivity and promotes triple-negative breast cancer development via the miR-1200/TPR axis.

Periodicals：iScience

IF：5.800

Article：CircZCCHC2 decreases pirarubicin sensitivity and promotes triple-negative breast cancer development via the miR-1200/TPR axis. 

Periodicals：iScience

IF：4.600

Article： Long noncoding RNA SNHG1 promotes breast cancer progression by regulating the miR-641/RRS1 axis.

Periodicals：Scientific reports

IF：4.600

Article：Circ_0070934 promotes MGAT3 expression and inhibits epithelial-mesenchymal transition in bronchial epithelial cells by sponging miR-199a-5p. 

Periodicals：Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology

IF：2.600

【2023年】

Article：A novel circular RNA, circMAML3, promotes tumor progression of prostate cancer by regulating miR-665/MAPK8IP2 axis.

Periodicals：Cell Death Discovery

IF：7.000

Article：CircPLXNA2 Affects the Proliferation and Apoptosis of Myoblast through circPLXNA2/gga-miR-12207-5P/MDM4 Axis

Periodicals：International Journal of Molecular Sciences

IF：6.208

Article：A novel circular RNA, circMAML3, promotes tumor progression of prostate cancer by regulating miR-665/MAPK8IP2 axis.

Periodicals：Cell death discovery

IF：6.100

Article：Integrated analysis identifies microRNA-188-5p as a suppressor of AKT/mTOR pathway in renal cancer

Periodicals：Cancer Science

IF：5.699

Article：LncRNA ANRIL promotes HR repair through regulating PARP1 expression by sponging miR-7-5p in lung cancer

Periodicals：BMC Cancer

IF：4.638

【2022年】

Article：Long noncoding RNA TINCR facilitates hepatocellular carcinoma progression and dampens chemosensitivity to oxaliplatin by regulating the miR-195-3p/ST6GAL1/NF-κB pathway

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.16

Article：MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4

Periodicals：Cell

IF：7.666

Article：CircDCLRE1C Regulated Lipopolysaccharide-Induced Inflammatory Response and Apoptosis by Regulating miR-214b-3p/STAT3 Pathway in Macrophages

Periodicals：International Journal of Molecular Sciences

IF：6.208

【2021年】

Article：CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression

Periodicals：Genes

IF：4.096