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實驗原理：

TRAP方法首先設計GST-MS2融合表達載體和ncRNA-MS2莖環結構串聯重復載體，共轉細胞獲得GST-MS2融合蛋白和ncRNA-MS2融合RNA，在胞內形成GST-MS2~ncRNA-MS2與蛋白或RNA的復合體；然后裂解細胞，利用谷胱甘肽親和瓊脂糖磁珠拉取獲得目的蛋白-RNA復合物；最后進一步分離純化，獲得目的蛋白或目的RNA進行下一步檢測分析。

 

產品優勢：

1. 伯信獨立研發。

2. 解決了傳統的TRAP實驗目前只應用于LncRNA等互作RNA或蛋白研究的局限性問題。

3. 使用環狀RNA表達載體而非探針進行雜交，避免了circRNA研究中接頭序列被蛋白結合后探針不能結合circRNA導致的假陰性影響，且降低了探針雜交過程中RNA的降解。

技術流程：

結果實例

詳見產品說明書：Bes5106 TRAP 

文章案例

Article： o8G-modified circPLCE1 inhibits lung cancer progression via chaperone-mediated autophagy. 

Periodicals：Molecular Cancer

IF：27.700

Article：m5C-modified circRREB1 promotes lung cancer progression by inducing mitophagy.

Periodicals：Journal of Experimental & Clinincal Cancer Research: CR

IF：12.800

Article：o8G-modified circKIAA1797 promotes lung cancer development by inhibiting cuproptosis. 

Periodicals：Journal of Experimental & Clinincal Cancer Research: CR

IF：12.800

Article： Long noncoding RNA semaphorin 6A-antisense RNA1 reduces hepatocellular carcinoma by promoting semaphorin 6A mRNA degradation.

Periodicals：World Journal of Gastroenterology

IF：4.300

Article：LncRNA FTX accelerates the progression of hepatocellular carcinoma by FTX/miR-374a-3p/HMGB1 pathway.

Periodicals：International Journal of Medical Sciences

IF：3.200

Article：MRTX1133 attenuates KRASG12D mutated-colorectal cancer progression through activating ferroptosis activity via METTL14/LINC02159/FOXC2 axis.

Periodicals：Translational Oncology

IF：4.500

Article：Circ6834 suppresses non-small cell lung cancer progression by destabilizing ANHAK and regulating miR-873-5p/TXNIP axis.

Periodicals：Molecular Cancer

IF：27.700

Article：CircNUP50 is a novel therapeutic target that promotes cisplatin resistance in ovarian cancer by modulating p53 ubiquitination.

Periodicals：Journal of nanobiotechnology

IF：10.200

Article：TRMT10C-mediated m7G modification of circFAM126A inhibits lung cancer growth by regulating cellular glycolysis.

Periodicals：Cell Biology and Toxicology

IF：5.300

Article：circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer

Periodicals：Molecular Cancer

IF：37.299

Article：LINC02159 promotes non-small cell lung cancer progression via ALYREF/YAP1 signaling

Periodicals：Molecular Cancer

IF：37.299

Article：Circular RNA EIF4G3 suppresses gastric cancer progression through inhibition of β-catenin by promoting δ-catenin ubiquitin degradation and upregulating SIK1

Periodicals：Molecular Cancer

IF：37.299

Article：ALKBH5-mediated m6A modifcation of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：Circular RNA circLAMA3 inhibits the proliferation of bladder cancer by directly binding an mRNA

Periodicals：Molecular Therapy: Oncolytics

IF：5.700

Article：Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis

Periodicals：Cell Biology and Toxicology

IF：6.100

Article：CircDIDO1 inhibits gastric cancer progression by encoding a novel DIDO1-529aa protein and regulating PRDX2 protein stability

Periodicals：Molecular Cancer

IF：37.300