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實驗原理：

細胞在非變性條件下被裂解時，細胞內許多“蛋白質-蛋白質”相互作用復合體被完整保留下來。利用目標蛋白質的特異性抗體進行免疫沉淀，再通過變性凝膠電泳分離所得蛋白質復合體，以此定性或定量分析幾種蛋白質之間的相互作用力。

 

產品優勢：

1. 伯信獨立研發，具有自主知識產權。

2. 配備完整：該試劑盒包含交聯劑，可使抗體與瓊脂糖珠交聯，洗脫時只收集誘餌蛋白和捕獲蛋白。

3. 同一個抗體既可用于免疫沉淀，又可用于WB檢測。

4. 操作簡單、耗時短、結果客觀準確。

技術流程

詳見產品說明書：Bes3011 Co-IP 

文章案例

Article：Elevated Dickkopf 3 Promotes Abdominal Aortic Aneurysm Formation via Modulated Phenotype Switch of Vascular Smooth Muscle Cells.

Periodicals：Research（Washington,D.C.）

IF：10.700

Article：Reduced Wiskott-Aldrich syndrome protein expression in preeclampsia placenta impairs trophoblast syncytialization by modulating syncytin-2 via FAK/β-catenin pathway. 

Periodicals：Frontiers in Cell and Developmental Biology

IF：4.300

Article：SIRT7 ameliorates Th17/Treg imbalance by desuccinylation of STAT3 to improve immune thrombocytopenia.

Periodicals：Clinical & Translational Immunology

IF：3.800

Article：m5C-modified circRREB1 promotes lung cancer progression by inducing mitophagy.

Periodicals：Journal of Experimental & Clinincal Cancer Research: CR

IF：12.800

Article：Lactate accumulation induces H4K12la to activate super-enhancer-driven RAD23A expression and promote niraparib resistance in ovarian cancer.

Periodicals：Molecular Cancer

IF：27.700

Article： o8G-modified circPLCE1 inhibits lung cancer progression via chaperone-mediated autophagy. 

Periodicals：Molecular Cancer

IF：27.700

Article：CircAKT3 promotes prostate cancer proliferation and metastasis by enhancing the binding of RPS27A and RPL11.

Periodicals：Molecular Cancer

IF：27.700

Article：LncRNA-MEG3 Regulates Muscle Mass and Metabolic Homeostasis by Facilitating SUZ12 Liquid-Liquid Phase Separation. 

Periodicals：Advance Science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.100

Article：GILT stabilizes cofilin to promote the metastasis of prostate cancer.

Periodicals：Cell death discovery

IF：7.000

Article：Exosome-derived hsa_circ_0007132 promotes lenvatinib resistance by inhibiting the ubiquitin-mediated degradation of NONO.

Periodicals：Non-coding RNA Research

IF：4.700

Article：Exosomal AFAP1-AS1 Promotes the Growth, Metastasis, and Glycolysis of Pituitary Adenoma by Inhibiting HuR Degradation.

Periodicals：Molecular Neurobiology

IF：4.600

Article：Circular RNA circ_0004470 accelerates the occurrence of lung cancer by promoting DNA damage and cell cycle arrest.

Periodicals：The Journal of Biological Chemistry

IF：4.000

Article： NSUN5 promotes cholangiocarcinoma progression by enhancing GLS mRNA stabilization.

Periodicals：Journal of Cancer Research and Clinical Oncology

IF：2.700

Article：Hepatocyte-derived Fetuin-A promotes alcohol-associated liver disease in mice by inhibiting autophagy-lysosome degradation of TLR and M2 macrophage polarization. 

Periodicals：Free Radical Biology & Medicine

IF：7.100

Article：The long noncoding RNA ELFN1-AS1 promotes gastric cancer growth and metastasis by interacting with TAOK1 to inhibit the Hippo signaling pathway.

Periodicals：Cell death discovery

IF：6.100

Article：circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA. 

Periodicals：Journal of Translational Medicine

IF：6.100

Article：HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells. 

Periodicals：Cell biology and Toxicology

IF：5.300

Article：TRMT10C-mediated m7G modification of circFAM126A inhibits lung cancer growth by regulating cellular glycolysis.

Periodicals：Cell Biology and Toxicology

IF：5.300

Article：Ubiquitination of angiotensin-converting enzyme 2 contributes to the development of pulmonary arterial hypertension mediated by neural precursor cell-expressed developmentally down-regulated gene 4-Like.

Periodicals：Respiratory Research

IF：4.700

Article： GBA3 promotes fatty acid oxidation and alleviates non-alcoholic fatty liver by increasing CPT2 transcription. 

Periodicals：Aging

IF：3.900

Article：CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling

Periodicals：Translational Research

IF：10.170

Article：The short inverted repeats-induced circEXOC6B inhibits prostate cancer metastasis by enhancing the binding of RBMS1 and HuR

Periodicals：Molecular Therapy

IF：12.400

Article：5mC and H3K9me3 of TRAF3IP2 promoter region accelerates the progression of translocation renal cell carcinoma

Periodicals：Biomarker Research

IF：11.100

Article：PAK6 promotes homologous-recombination to enhance chemoresistance to oxaliplatin through ATR/CHK1 signaling in gastric cancer

Periodicals：Cell Death and Disease

IF：9.000

Article：Coordinated transcriptional and post-transcriptional epigenetic regulation during skeletal muscle development and growth in pigs

Periodicals：Journal of Animal Science and Biotechnology

IF：7.000

Article：Transcription factor ZNF22 regulates blood-tumor barrier permeability by interacting with HDAC3 protein

Periodicals：Frontiers in Molecular Neuroscience

IF：4.800

Article：Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease

Periodicals：Frontiers in Pharmacology

IF：5.600

Article：MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds

Periodicals：Scientific Reports

IF：4.600