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實驗原理：

ChIRP方法通過設計生物素標記RNA互補探針，并使其與鏈霉親和素結合，這樣，在探針與目標RNA特異性結合的同時，捕獲RNA結合調控的DNA染色體片段與參與轉錄調控的RNA結合蛋白質（RBPs）。

DNA染色體片段經過文庫構建與高通量測序，在基因組水平上獲得轉錄調控RNA（增強子RNA, eRNA）調控的下游靶基因，結合qPCR可以研究結合調控強度； RNA結合蛋白經過酶消化與高效液相-質譜分析，可以鑒定參與轉錄調控的蛋白質，結合Western Blot可以進一步研究結合作用強度。

 

產品優勢：

1. 伯信獨立研發，具有自主知識產權。

2. 特異性和靈敏度高，穩定性好。

3. 檢測方法領先，結果準確、重復性好。

4. 快速檢測，操作簡單，安全便捷。

技術路線：

結果實例：

詳見產品說明書：Bes5104-1 ChIRP-DNA

詳見產品說明書：Bes5104-2 ChIRP-Protein

詳見產品說明書：Bes5104-3 ChIRP-DNA、Protein

注：Bes5104-3 ChIRP-DNA&Protein （同時拉RNA結合DNA和蛋白）的用戶可根據實驗目的自行參考說明書Bes5104-1 ChIRP-DNA（只研究RNA結合DNA） 或Bes5104-2 ChIRP-Protein （只研究RNA結合蛋白）進行實驗操作。 

文章案例

【2025年】

Article：Enhancer transcription profiling reveals an enhancer RNA-driven ferroptosis and new therapeutic opportunities in prostate cancer. 

Periodicals：Signal Transduction and Targeted Therapy

IF：40.800

Article：Intranuclear paraspeckle-circular RNA TACC3 assembly forms RNA-DNA hybrids to facilitate MASH-related hepatocellular carcinoma growth in an m6A-dependent manner. 

Periodicals：Cancer Communications (London, England)

IF：24.900

Article：Gain of Alternative Allele Expression of LINC02449 at rs149707223 in Schizophrenia and Bipolar Disorder: Inducing Synaptic Transmission and Behavioral Deficits in Mice.

Periodicals：Nature Communications

IF：15.700

Article：o8G-modified circKIAA1797 promotes lung cancer development by inhibiting cuproptosis. 

Periodicals：Journal of Experimental & Clinincal Cancer Research: CR

IF：11.400

Article：MIAT promotes tumor-infiltrating CD8+ T-cell exhaustion and malignant progression of renal cell carcinoma via activating JAK3/STAT3 pathway. 

Periodicals：Journal for Immunotherapy of Cancer

IF：10.600

【2024年】

Article： Genome-wide enhancer RNA profiling adds molecular links between genetic variation and human cancers.

Periodicals：Military Medical Research

IF：16.700

Article：CircUGP2 Suppresses Intrahepatic Cholangiocarcinoma Progression via p53 Signaling Through Interacting With PURB to Regulate ADGRB1 Transcription and Sponging miR-3191-5p. 

Periodicals：Advanced Science

IF：14.300

Article：M6A-mediated upregulation of lncRNA TUG1 in liver cancer cells regulates the antitumor response of CD8+ T cells and phagocytosis of macrophages. 

Periodicals：Advanced Science

IF：14.300

Article： LINC00606 promotes glioblastoma progression through sponge miR-486-3p and interaction with ATP11B. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：Positive feedback loop of c-myc/XTP6/NDH2/NF-κB to promote malignant progression in glioblastoma. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：Fumarate induces LncRNA-MIR4435-2HG to regulate glutamine metabolism remodeling and promote the development of FH-deficient renal cell carcinoma.

Periodicals：Cell death & Disease

IF：8.100

Article：Gain of bipolar disorder-related lncRNA AP1AR-DT in mice induces depressive and anxiety-like behaviors by reducing Negr1-mediated excitatory synaptic transmission.

Periodicals：BMC Medicine

IF：7.000

Article： CircARAP2 controls sMICA-induced NK cell desensitization by erasing CTCF/PRC2-induced suppression in early endosome marker RAB5A.

Periodicals：Cellular and Molecular Life Sciences

IF：6.200

Article：EIF4A3-regulated hsa_circ_0001445 can inhibit the progression of laryngeal squamous cell carcinoma via hsa-miR-432-5p-dependent up-regulation of RGMA expression. 

Periodicals：Cell cycle (Georgetown, Tex.)

IF：3.400

【2023年】

Article：hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma.

Periodicals：Molecular Cancer

IF：37.2999

Article：M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：Long Non-Coding RNA BNIP3 Inhibited the Proliferation of Bovine Intramuscular Preadipocytes via Cell Cycle

Periodicals：International Journal of Molecular Sciences

IF：5.600

Article：Lnc-LRRTM4 promotes proliferation, metastasis and EMT of colorectal cancer through activating LRRTM4 transcription

Periodicals：Cancer Cell International

IF：5.800

【2022年】

Article：5mC and H3K9me3 of TRAF3IP2 promoter region accelerates the progression of translocation renal cell carcinoma

Periodicals：Biomarker Research

IF：11.100

Article：LncRNA HOXA11-AS promotes glioma malignant phenotypes and reduces its sensitivity to ROS via Tpl2-MEK1/2-ERK1/2 pathway

Periodicals：Cell Death and Disease

IF：9.000

Article： LINC01615 maintains cell survival in adaptation to nutrient starvation through the pentose phosphate pathway and modulates chemosensitivity in colorectal cancer.

Periodicals：Cellular and molecular life sciences

IF：6.200

Article：LncRNA FENDRR with m6A RNA methylation regulates hypoxia-induced pulmonary artery endothelial cell pyroptosis by mediating DRP1 DNA methylation

Periodicals：Molecular Medicine

IF：5.700

【2021年】

Article：Downregulation of microRNA-6125 promotes colorectal cancer growth through YTHDF2-dependent recognition of N6-methyladenosine-modified GSK3β

Periodicals：Clinical and Translational Medicine

IF：10.600

Article：Upregulated LINC01667 Expression Is Correlated With Poor Prognosis in Hepatocellular Carcinoma

Periodicals：Frontiers in Oncology

IF：6.244

【2020年】

Article：Long Noncoding RNA MRPL23-AS1 Promotes Adenoid Cystic Carcinoma Lung Metastasis

Periodicals：Cancer Research

IF：11.200

Article：Super-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

Periodicals：iScience

IF：4.447

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced Science

IF：17.521

【2017年】

Article：LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1

Periodicals：Journal of Cellular and Molecular Medicine

IF：4.302