實驗原理：
RAP技術通過設計生物素探針組拉取目標RNA，使與其共同作用的RNA或蛋白質(zhì)（RBPs）富集在磁珠上并被洗脫。最后，通過高通量測序得到該調(diào)控RNA轉(zhuǎn)錄調(diào)控的下游靶基因，同時，也可以通過Western Blot驗證RBPs，或通過MS鑒定未知蛋白。
產(chǎn)品優(yōu)勢：
1. 伯信獨立研發(fā)，具有自主知識產(chǎn)權(quán)。
2. 靈敏度高，穩(wěn)定性好。
3. 檢測方法領先，結(jié)果準確、重復性好。
技術路線：
結(jié)果案例：
詳見產(chǎn)品說明書：Bes5103-1 RAP-RNA
詳見產(chǎn)品說明書：Bes5103-2 RAP-Protein
詳見產(chǎn)品說明書：Bes5103-3 RAP-RNA Protein
注：Bes5103-3 RAP-RNA&Protein （同時拉RNA結(jié)合RNA和蛋白）的用戶可根據(jù)實驗目的自行參考說明書Bes5103-1 RAP-RNA（只研究RNA結(jié)合RNA） 或Bes5103-2 RAP-Protein （只研究RNA結(jié)合蛋白）進行實驗操作。
文章案例
【2025年】
Article：Targeting cTRIP12 counteracts ferroptosis resistance and augments sensitivity to immunotherapy in pancreatic cancer.
Periodicals：Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy
IF：15.800
Article：Identification of a JAK–STAT–miR155HG positive feedback loop in regulating natural killer (NK) cells proliferation and effector functions
Periodicals：Acta Pharmaceutica Sinica B
IF：14.700
Article：SenExo-cCCT2 Reprograms Senescence Response and Anti-Tumor Immunity Following FOLFIRINOX Chemotherapy in Pancreatic Ductal Adenocarcinoma.
Periodicals：Advanced Science (Weinheim, Baden-Wurttemburg, Germany)
IF：14.100
Article：CircTP53/USP10/p53 signaling Axis as a Novel Regulator of Progression and Prognosis of Head and Neck Squamous Cell Carcinoma.
Periodicals：Advanced Science (Weinheim, Baden-Wurttemburg, Germany)
IF：14.100
Article：Cuticle protein mediates the evolution of stress resistance by generating a decoy circular RNA in spider mite.
Periodicals：Science Advances
IF：12.500
Article: N6-methyladnosine of vRNA facilitates influenza A virus replication by promoting the interaction of vRNA with polymerase proteins.
Periodicals：Proceedings of the National Academy of Sciences of the United States of America
IF：9.100
Article: CSF3R-AS promotes hepatocellular carcinoma progression and sorafenib resistance through the CSF3R/JAK2/STAT3 positive feedback loop.
Periodicals：Cell Death & Disease
IF：8.100
Article：piRNA28846 has the potential to be a novel RNA nucleic acid drug for ovarian cancer.
Periodicals：NPJ Precision Oncology
IF：6.800
Article：Nucleophosmin 1 inhibits the replication of influenza A virus by competitively binding viral RNA with viral proteins.
Periodicals：Virological Sinica
IF：4.000
Article：circSLC39A8 by sponging hsa-miR-11181-5p and direct binding to PIK3CA mRNA promotes retinoblastoma proliferation.
Periodicals：Journal of Cancer
IF：3.200
【2024年】
Article： Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer.
Periodicals：Molecular cancer
IF：27.700
Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.
Periodicals：Molecular cancer
IF：27.700
Article：Escherichia coli-Induced cGLIS3-Mediated Stress Granules Activate the NF-κB Pathway to Promote Intrahepatic Cholangiocarcinoma Progression.
Periodicals：Advanced Science
IF：14.300
Article：circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis.
Periodicals：Redox biology
IF：10.700
Article：Blockade of the lncRNA-PART1-PHB2 axis confers resistance to PARP inhibitor and promotes cellular senescence in ovarian cancer.
Periodicals：Cancer Letters
IF：9.100
Article：CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA.
Periodicals：Cell death & disease
IF：8.100
Article：circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA.
Periodicals：Journal of Translational Medicine
IF：6.100
Article：Three-dimensional cultured human umbilical cord mesenchymal stem cells attenuate pulmonary fibrosis by improving the balance of mitochondrial fusion and fission.
Periodicals：Stem Cells Translational Medicine
IF：5.400
Article：circ-1584 selectively promotes the antitumor activity of the oncolytic virus M1 on pancreatic cancer.
Periodicals：Molecular Therapy. Oncology
IF：5.299
Article：circRUNX2.2, highly expressed in Marek's disease tumor tissues, functions in cis to regulate parental gene RUNX2 expression.
Periodicals：Poultry Science
IF：3.800
【2023年】
Article：N6-methyladenosine-modified circRIMS2 mediates synaptic and memory impairments by activating GluN2B ubiquitination in Alzheimer's disease.
Periodicals：Translational Neurodegeneration
IF：12.600
Article：METTL3/YTHDC1-medicated m6A modification of circRNA3634 regulates the proliferation and differentiation of antler chondrocytes by miR-124486-5-MAPK1 axis.
Periodicals：Cellular & molecular biology letters
IF：9.200
Article：Circular RNA circ0001955 promotes cervical cancer tumorigenesis and metastasis via the miR-188-3p/NCAPG2 axis
Periodicals：Journal of Translational Medicine
IF：7.400
Article： N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomes
Periodicals：iScience
IF：5.800
Article：circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization
Periodicals：iScience
IF：5.800
Article：hucMSCs treatment prevents pulmonary fibrosis by reducing circANKRD42-YAP1-mediated mechanical stiffness.
Periodicals：Aging (Albany NY)
IF：5.200
Article：lhCLIP reveals the in vivo RNA-RNA interactions recognized by hnRNPK
Periodicals：PloS Genetics
IF：4.500
Article：LincRNA00612 inhibits apoptosis and inflammation in LPS-induced BEAS-2B cells via enhancing interaction between p-STAT3 and A2M promoter
Periodicals：PeerJ
IF：2.700
【2022年】
Article：The N6-methyladenosine modifcation of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway
Periodicals：Molecular Cancer
IF：37.300
Article：EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathway
Periodicals：Journal of Experimental & Clinical Cancer Research
IF：11.300
Article：LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2
Periodicals：Cell Death and Disease
IF：9.000
Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition
Periodicals：Cell Death and Disease
IF：8.469
Article：The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by bindingmRNA and regulating its stability
Periodicals：Molecular Therapy: Oncolytics
IF：6.311
Article：Long Non?coding Antisense RNA DDIT4?AS1 Regulates Meningitic Escherichia coli?Induced Neuroinfammation by Promoting DDIT4 mRNA Stability
Periodicals：Molecular Neurobiology
IF：5.59
Article：Long non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection
Periodicals：Molecular Brain
IF：4.04
【2021年】
Article：LINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation
Periodicals：Bioengineering & Translational Medicine
IF：7.400
Article：Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis
Periodicals：Cell Death & Disease
IF：8.469
Article：Circular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression
Periodicals：Cell Death & Disease
IF：8.469
Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition
Periodicals：Cell Death & Disease
IF：8.469
Article：Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression
Periodicals：Cell Death & Disease
IF：8.469
Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity
Periodicals：Cell Death & Disease
IF：8.469
Article：lncRNA GAS5, as a ceRNA, inhibits the proliferation of diffuse large B?cell lymphoma cells by regulating the miR?18a?5p/RUNX1 axis
Periodicals：INTERNATIONAL JOURNAL OF ONCOLOGY
IF：5.65
Article：CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5
Periodicals：Frontiers in Molecular Neuroscience
IF：5.630
Article：uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation
Periodicals：Frontiers in Oncology
IF：4.848
Article：CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression
Periodicals：Cancer Cell International
IF：4.175
Article：lncRNA RP11?531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression
Periodicals：Experimental and Therapeutic Medicine
IF：2.447
【2020年】
Article：circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.800
Article：CircRNA104250 and lncRNAuc001.dgp.1 promote the PM2.5-induced inflammatory response by co-targeting miR-3607-5p in BEAS-2B cells
Periodicals：Environmental Pollution
IF：6.792
Article：The regulatory network among CircHIPK3, LncGAS5, and miR-495 promotes Th2 differentiation in allergic rhinitis
Periodicals：Cell Death & Disease
IF：6.302
【2019年】
Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family
Periodicals：Advanced science
IF：15.100
Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer
Periodicals：Molecular Cancer
IF：7.776
Article：CircPCMTD1 Acts as the Sponge of miR-224-5p to Promote Glioma Progression
Periodicals：Frontiers in Oncology
IF：4.137
Article：Mechanism of aberrant long non-coding RNA expression in an adriamycin-resistant liver cancer cell strain
Periodicals：Digestive and Liver Disease
IF：4.088
【2016年】
Article：A novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis
Periodicals：Archives of Toxicology
IF：5.728
